OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like carbapenemase gene PCR
Registry Details
Area | Health Science |
Lab name | Antibiotic Reference Laboratory |
Lab location | Kenepuru Science Centre, Porirua |
Test name | OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like carbapenemase gene PCR |
Organisms | Acinetobacter and Pseudomonas |
Requested for surveillance | no |
Notice required | no |
Routine turnaround time | 6 working days for reference specimens |
Culture requirements | Pure culture |
Media requirements | A slope of any medium that supports the growth of the organism |
Pre-transport requirements | Ambient temperature |
Transport requirements | Ambient temperature |
Unacceptable sample types | Clinical specimens will not be accepted. |
How results reported | The results will state whether OXA-23-like, OXA-40-like, OXA-58-like or OXA-51-like genes or the ISAba1 insertion sequence were detected |
Referral link | Form |
Details
Demonstration of the presence of an OXA carbapenemase gene by PCR.
Carbapenemase-mediated carbapenem resistance in Acinetobacter is most often due to the production of an OXA-23-like, OXA-40-like or OXA-58-like carbapenemase. In addition, carbapenem resistance in Acinetobacter may be due to overexpression of an OXA-51-like carbapenemase. OXA-51-like carbapenemases are intrinsic in A. baumannii and normally expressed at low levels but they can be overexpressed following the insertion of an ISAba1 sequence element upstream of the OXA-51 gene.
To check for the presence of OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like genes, a multiplex PCR is used and contains primers for each of the four groups of OXA-type carbapenemases. Any Acinetobacter isolates that are carbapenem resistant, but only positive for OXA-51-like genes in the multiplex PCR, are tested by PCR for the ISAba1 insertion sequence.
This test is appropriate for carbapenem-resistant Acinetobacter or Pseudomonas isolates.