Plasmid-mediated AmpC β-lactamase gene PCR
Registry Details
Area | Health Science |
Lab name | Antibiotic Reference Laboratory |
Lab location | Kenepuru Science Centre, Porirua |
Test name | Plasmid-mediated AmpC β-lactamase gene PCR |
Organisms | Escherichia coli, Klebsiella spp and Salmonella spp |
Requested for surveillance | no |
Notice required | no |
Routine turnaround time | 10 working days for reference specimens |
Culture requirements | Pure culture |
Media requirements | A slope of any medium that supports the growth of the organism |
Pre-transport requirements | Ambient temperature |
Transport requirements | Ambient temperature |
Unacceptable sample types | Clinical specimens will not be accepted. |
How results reported | The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected |
Referral link | Form |
Details
Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.
The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.