IMP, VIM, NDM, SIM, GES and IMI-carbapenemase gene PCR
Registry Details
Area | Health Science |
Lab name | Antibiotic Reference Laboratory |
Lab location | Kenepuru Science Centre, Porirua |
Test name | IMP, VIM, NDM, SIM, GES and IMI-carbapenemase gene PCR |
Organisms | Enterobacterales, Pseudomonas and Acinetobacter |
Requested for surveillance | yes |
Notice required | no |
Routine turnaround time | 6 working days for reference specimens |
Culture requirements | Pure culture |
Media requirements | A slope of any medium that supports the growth of the organism |
Pre-transport requirements | Ambient temperature |
Transport requirements | Ambient temperature |
Unacceptable sample types | Clinical specimens will not be accepted. |
How results reported | The results will state whether IMP, VIM or NDM genes were detected |
Referral link | Form |
Details
Demonstration of the presence of IMP, VIM or NDM metallo-β-lactamase genes by PCR. The test is appropriate for ceftazidime-resistant Enterobacterales, Pseudomonas and Acinetobacter with carbapenem resistance or reduced susceptibility.
Prior to performing the PCR, ESR will screen the isolate for metallo-β-lactamase using the CLSI modified carbapenem inactivation method, and EDTA and MPA/TE inhibitor-based double-disc synergy tests.